Brassinolide-mediated defense responses in Hordeum vulgare L. against Fusarium culmorum: genetic, epigenetic, and transcriptional insights
Journal of Plant Pathology, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Basım Tarihi: 2026
- Doi Numarası: 10.1007/s42161-026-02328-3
- Dergi Adı: Journal of Plant Pathology
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, Environment Index, Natural Science Collection (ProQuest), Biological Science Database (ProQuest), Biomedical Reference Collection: Corporate Edition (EBSCO)
- Anahtar Kelimeler: Antifungal activity, Biotic stress, Bisulfite sequencing, CRED-RA, IRAP
- İstanbul Yeni Yüzyıl Üniversitesi Adresli: Evet
Özet
In this project, the potential of brassinolide (BR) to mitigate the necrotic effects of Fusarium culmorum on barley (Hordeum vulgare L.) roots and the underlying molecular mechanisms of this effect were investigated. Two barley genotypes, relatively resistant (H. vulgare L. cv. Avcı-2002) and susceptible (H. vulgare L. cv. Larende) to F. culmorum, were used. In pathogenicity tests, the experimental groups were consisted of an uninfected control group, a macroconidium-only infected group, and BRs (0.1, 0.5, and 1 µM) treatment groups. In both genotypes, a 0.5 µM BR treatment had positive effects on root length, root number, and disease severity; infection severity decreased by 24%, and plant growth was comperable to that of the control group (p > 0.05). In BAGY2 and BARE1 transposon analyses, the similarity ratios of transposons were between 75.7% and 100% and 84.8%-100% and a relatively high level of transposon mobility was detected. Genomic template stability (GTS) by CRED-RA yielded values between 78.82% and 94.44%, and Avcı-2002 genotype became relatively more stable with the BRs application. Intragenic methylations and transcriptional changes in the BAGY2, NadD, and WRKY6 genes showed contrast profiles in infected and BRs-treated samples in gene-specific methylation and gene expression. In the global methylation analysis, total methylation levels increased in the infected and BRs-treated groups. These findings suggest that BR application may have a very low level of plant protective effect, which can be confirmed by genomic, epigenomic, and transcriptional methods. Further studies can be supported by holistic transcript analyses.