Antiproliferative constituents from the aerial parts of Chrysophthalmum montanum (DC.) Boiss

Ayaz F., EMERCE E., Gören N., Çalış İ., Ur Rehman M., Iqbal Choudhary M., ...Daha Fazla

Phytochemistry Letters, cilt.36, ss.173-182, 2020 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 36
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1016/j.phytol.2020.01.003
  • Dergi Adı: Phytochemistry Letters
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, EMBASE, Veterinary Science Database
  • Sayfa Sayıları: ss.173-182
  • Anahtar Kelimeler: Antiproliferative effect, Chrysophthalmum montanum, MTT assay, Sesquiterpene lactone
  • İstanbul Yeni Yüzyıl Üniversitesi Adresli: Evet

Özet

Three undescribed sesquiterpene lactones (1-3), 1β,4β-dihydroxy-guaia-10(14),11(13)-dien-8α,12-olide (1), 4α, 6α-dihydroxy-9β,10β-epoxy-1βH-guaia-11(13)-en-8α,12-olide (2), and 4α,9β-dihydroxy-6α-acetoxy-1βH-guaia-10(14),11(13)-dien-8α,12-olide (3), as well as a known sesquiterpene lactone, (4α,5α,8β,10α)-4,10-dihydroxy-1,11(13)-guaiadien-12,8-olide (4), along with a lignan, pinoresinol (5), a flavonoid, chrysosplenol C (6), and two triterpenes, a mixture of isomers, taraxasterol acetate and Ψ-taraxasterol acetate (7a/7b), and a mixture of isomers taraxasterol and Ψ-taraxasterol (8a/8b) were isolated from the aerial parts of Chrysophthalmum montanum (DC.) Boiss. The structures of 1-6, 7a/7b, and 8a/8b were established on the basis of spectroscopic evidence, such as MS, NMR, UV, and IR spectroscopy. All isolated compounds, except for 5 and 8a/8b, were assayed for their antiproliferative activities against three human cancer cell lines, i.e. cervical (HeLa), breast (MCF-7), and lung (A549), and a normal human lung cell line (BEAS-2B) using MTT method. Compounds 1, 4, and 6 showed significant inhibitory effects on cancer cell growth at 20 μg/mL concentration, with cell viability ranging from 53 to 64 % against MCF-7 cell line. In addition, compounds 4, and 6 exhibited cytotoxicity against HeLa cancer cell line with the viability of approximately 64 %. In conclusion, compounds 1, 4, and 6 may serve as leads for further research towards the development of anticancer agents.