Akademik Veri Yönetim Sistemi
Turk Hijyen ve Deneysel Biyoloji Dergisi, cilt.77, sa.Supplement 4, ss.71-76, 2020 (Scopus)
Objective: Helicobacter pylori is a bacterium which colonizes the human gastric mucosa and known to affect half of the world’s population. H. pylori is defined as the aetiological agent of peptic ulcer, gastric adenocarcinoma and mucosa-associated lymphoid tissue lymphoma. However, the natural host and reservoir have not been clearly identified. The ability of adaptation and biofilm formation, which play an important role in the virulence of the bacterium, constituted the basis of studies about water contamination. In relation with this, bacterial DNA was detected in drinking water, surface water, groundwater and wastewater in many studies.It has been suggested that the transmission of bacterium occurs via fecal-oral, oraloral and gastro-oral routes, additionally contaminated foods and water may be source of infection. In the present preliminary study, it was aimed to investigate the presence of H. pylori DNA in drinking water samples. Methods: Thirty five different trademarked drinking water samples (each one 500 mL) were collected from March- June 2019 in all around Istanbul. It was observed that water samples have a slightly alkaline pH ranging from 6.6 to 8.45. All water samples were filtrated using 0.22 μM filter membranes which were incubated on Brain Heart Infusion (BHI) broth for half hour at room temperature and filter included broths were centrifuged. Then bacterial DNA was extracted. Polymerase Chain Reaction (PCR) was used for the detection of the ureC (glmM) gene. The PCR products were visualised in %1.5 agarose gel electrophoresis. Results: In this study H. pylori DNA was not detected in any of the water samples tested. Conclusion: This preliminary study results have suggested that, drinking waters do not have a role in transmission of H. pylori in this geographical area. However, it is important to carry out more extensive studies since the number of samples examined in this study was limited and only bottles sold in 500 mLpackaging are included. Therefore it may be concluded that different water sources such as 19L flagon bottled drinking waters, tap waters and water samples from various tanks may be useful for clarifying the role of water for transmission route of this pathogen.