Mitogenome targeted phylogenetic analysis in fusarium head blight pathogens

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Yörük E.

3. Bilsel International Efes Scientific Researches and Innovatıon Congress, İzmir, Türkiye, 22 Mart 2024, ss.349, (Özet Bildiri)

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: İzmir
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.349
  • İstanbul Yeni Yüzyıl Üniversitesi Adresli: Evet

Özet

Fusarium head blight (FHB) is one of the most devastating diseases of cereals worldwide. Genomic plasticity

and the relatively high level of polymorphisms in chromosomal and mitochondrial genomes (mitogenome,

"mtDNA") of FHB pathogens are important reasons for failure to control FHB. In this study, mtDNA genome

based phylogenetic analysis were carried out in causal agents of FHB with totally 20 accessions (Fusarium

graminearum with 15 members, F. culmorum, F. avenaceum, F. pseudograminearum, F. poae, F. proliferatum,

and F. solani-F.oxysporum as outer groups). Nucleotide sequences of mtDNA were obtained from GenBank.

Sequence similarity and UPGMA trees were constructed by MAFFT software. R/RStudio (with packages of

"ape", "phangorn", and "factoextra") was used to obtain distance matrix, maximum likelihood (ML)

dendrogram, and principal component (PCA) analysis-derived data of FHB pathogens. Bayesian topology

analysis was carried out using MrBayes software via Ubuntu-Linux terminal. Individuals belonging to different

species showed markedly different clustering via ML and UPGMA dendrograms which included F.

graminearum members within the same sub-cluster. Distance matrix also supported this cluster profiling with

mean distance value as 41.75%. In PCA analysis, FHB pathogens were clustered together in Dim1 and Dim2

whereas F. solani and F.oxysporum (not FHB pathogens) were clustered distinctly. Percentages of dimensions

were as 93% and 6% for x and y axes meaning that FHB causal agents were genetically so similar for their

mtDNA phylogeny. Bayesian topology analysis showed that members of F. graminearum and remaining FHB

pathogens were genetically dissimilar with F. solani and F. oxysporum. It was found that mitogenome can

strongly differentiate Fusarium spp. not only at the species-specific level, but also in terms of pathogen

characteristics. Specific and potential marker genes located in mtDNA of FHB pathogens can also be

investigated in future studies for detailed characterization of FHB pathogens.