Akademik Veri Yönetim Sistemi
Asian Journal of Andrology, cilt.15, sa.4, ss.518-522, 2013 (SCI-Expanded)
We aimed to investigat. The role of thioredoxin reductase (TR) and inducible heat shock protein 70 (iHsp70) and their relationship with sperm quality in varicocele (VAR) patients. Semen samples were obtained from 16 subfertile men diagnosed as VAR and 10 fertile men who applied t. The Andrology Laboratory of Istanbul Medical Faculty of Istanbul University. The sperm TR and iHsp 70 expression levels were determined using Western blot analysis. The TR activity o. The sperm was assayed spectrophometrically. The sperm quality was evaluated both by conventional sperm analysis and by a terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) technique that assayed DNA-fragmented spermatozoa in semen samples. The percentage of TUNEL-positive spermatozoa i. The VAR group (16.3%±5.6%) was higher than that i. The fertile group (5.5%±1.9%). Significant inverse correlations were detected betwee. The percentage of TUNEL-positive cells and bot. The concentration (r=-0.609; P=0.001) and motility (r=-0.550; P=0.004) of spermatozoa. Bot. The TR expression and activity were increased significantly i. The VAR group (U=22.0; P=0.001 and U=33.5; P=0.012, respectively) as analyzed usin. The Mann-Whitney U Wilcoxon rank sum W test. Furthermore, significant positive correlations were found between TR expression and activity (r=0.406; P=0.040) and between TR expression an. The percentage of TUNEL-positive cells (r=0.665; P=0.001). Sperm iHsp70 expression did not differ betwee. The VAR and fertile groups. In conclusion, increased sperm TR expression might be a defense mechanism against apoptosis i. The spermatozoa of men with VAR.