Actin - An inhibitor of eukaryotic elongation factor activities

Bektaş M., Günçer B., Güven C., Nurten R., Bermek E.

Biochemical and Biophysical Research Communications, cilt.317, sa.4, ss.1061-1066, 2004 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 317 Sayı: 4
  • Basım Tarihi: 2004
  • Doi Numarası: 10.1016/j.bbrc.2004.03.153
  • Dergi Adı: Biochemical and Biophysical Research Communications
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1061-1066
  • Anahtar Kelimeler: Actin, ADP-ribosylation, Cytoskeleton, Eukaryotic elongation factors 1 and 2, Protein synthesis
  • İstanbul Yeni Yüzyıl Üniversitesi Adresli: Hayır

Özet

An inhibitor of diphtheria toxin- and endogenous transferase-dependent ADP-ribosylation of eukaryotic elongation factor 2 (eEF2) has been found in the cytoplasmic fraction from rat liver. We provide evidence that this cytoplasmic inhibitor corresponds to actin, which gives rise also to inhibition of polyphenylalanine (polyPhe) synthesis. Both globular monomeric (G-actin) and filamentous (F-actin) forms of actin appear to be inhibitory on the action of elongation factors 1 and 2 (eEF1 and eEF2) in polyPhe synthesis with the inhibitory effect of G-actin proving to be stronger. Some component(s) in the postribosomal supernatant (S-130) fraction and also DNase I prevent actin-promoted inhibition of polyPhe synthesis. © 2004 Elsevier Inc. All rights reserved.