Akademik Veri Yönetim Sistemi
31 st International Conference on Yeast Genetics and Molecular Biology, Florence, İtalya, 20 - 25 Ağustos 2023, ss.225, (Özet Bildiri)
Glucose plays key role as fundamental source of carbon and energy in eukaryotes, and its uptake are therefore vital to drive various energy-consuming cellular processes. Although it is known that two pathways (cAMP/PKA and MAPK) are involved in the sensing of glucose signal in Schizosaccharomyces pombe fission yeast, there is limited information about the regulation of hexose transporter genes (ght1-8) whose expression varies in different glucose concentrations and carbon sources. We aim to investigate the possible effect of SPCC320.03 transcription factor, which is thought to be involved in the pathway, on ght1-8 genes at different carbon sources and glucose concentration. For this purpose, we used parental and ΔSPCC320.03 strains of S. pombe and their transformants. Significant changes in the expression of ght3-6 were observed in SPCC320.03 overexpressed strain, ΔSPCC320.03, and its pSLF272- SPCC320.03 transformant grown on 2% gluconate. In SPCC320.03 over-expressed strain, ght3-6 the expressions were suppressed. The expression of ght3, ght4, ght6 increased in ΔSPCC320.03 compared to parental strain. In addition, the expressions of ght3-6 were suppressed in the transformant strains containing a single copy of SPCC320.03 gene, compared to the ΔSPCC320.03 strain. On the contrary, the expression of ght2 in SPCC320.03 overexpressed strain was regulated positively in the 2% glycerol. It has also been shown that expression of ght1, ght7 and ght8 genes may vary depending on carbon sources but is not affected by expression of SPCC320.03 gene. We suggested that DNA-binding transcription factor SPCC320.03 has a role in the regulation of ght3, gh4 and ght6 genes.