The effects of amrinone and glucagon on verapamil-induced myocardial depression in a rat isolated heart model


TUNÇOK Y., Apaydin S., Gidener S., GÜVEN H., Oto O., ATEŞ M., ...More

General Pharmacology, vol.28, no.5, pp.773-776, 1997 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 28 Issue: 5
  • Publication Date: 1997
  • Doi Number: 10.1016/s0306-3623(96)00093-6
  • Journal Name: General Pharmacology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.773-776
  • Keywords: amrinone, Calcium channel blocker, glucagon, rat isolated heart, verapamil toxicity
  • İstanbul Yeni Yüzyıl University Affiliated: No

Abstract

1. We measured the ability of glucagon and amrinone, used alone and in combination, to improve the myocardial function in a rat isolated heart model of calcium channel blocker (CCB) cardiotoxicity. 2. Verapamil 10-4 mol consistently decreased heart rate and cardiac contractile force in our Langendorff rat isolated heart preparations. Glucagon increased the heart rate in a dose-dependent fashion. Amrinone increased the heart rate only at the 1 x 10-3 mol concentration, and bad no significant effect on cardiac contractility. 3. A positive linear correlation was found between the glucagon concentration and the percent recovery of baseline contractile force. 4. Although complete reversal of verapamil-induced myocardial depression occurred at glucagon concentrations of >3x10-6 mol, amrinone produced only 23.8±3.6% recovery from baseline at its highest concentration (4x10 3 mol). 5. When glucagon and amrinone were administered together, there was no additional increase over glucagon alone in the increase in contractile force. 6. Glucagon, and not amrinone, is an appropriate agent, capable of reversing verapamil-induced myocardial toxicity in this rat isolated heart model. In vivo studies should be performed to assess whether this may be a reliable therapy in clinical cases.