The effect of pentylenetetrazole-induced seizures on blood-brain barrier integrity in a rat model of preeclampsia
Turkish Journal of Veterinary and Animal Sciences, cilt.36, sa.3, ss.311-318, 2012 (SCI-Expanded, Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 36 Sayı: 3
- Basım Tarihi: 2012
- Doi Numarası: 10.3906/vet-1108-2
- Dergi Adı: Turkish Journal of Veterinary and Animal Sciences
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.311-318
- Anahtar Kelimeler: Aquaporin-4, Hypertension, Occludin, Pregnancy, Sodium fluorescein
- İstanbul Yeni Yüzyıl Üniversitesi Adresli: Hayır
Özet
The pathophysiological mechanisms underlying blood-brain barrier (BBB) disruption in preeclampsia/eclampsia have not been elucidated. This study investigated the effect of pentylenetetrazole (PTZ)-induced seizures on the functional and structural properties of the BBB during N (omega)-nitro-L-arginine methylester (L-NAME)-induced hypertension and proteinuria in pregnant rats. Animals were treated with L-NAME for 10 days, beginning on day 10 of pregnancy. The BBB permeability was determined by measurements of sodium fluorescein (NaFlu)extravasation into the brain. Occludin and aquaporin (AQP)-4 immunoreactivities were evaluated in brain sections. Severe proteinuria and significantly increased arterial blood pressure were observed following L-NAME treatment. PTZinduced seizures in pregnant rats treated with L-NAME increased NaFlu levels in all of the brain regions analyzed (P <0.01).A significant increase in the extravasation of NaFlu was also observed in the diencephalon of intact pregnant rats treated with PTZ. PTZ-induced seizures in both L-NAME-treated and untreated pregnant rats significantly decreased occludin immunoreactivity in the hippocampal capillaries. L-NAME administration significantly increased AQP-4 immunoreactivity in the astrocytic endfeet surrounding the parietal cortex microvessels of PTZ-treated and untreated pregnant rats. These findings suggest that PTZ-induced seizures lead to a severe disruption of the BBB in preeclampsia and that the paracellular pathway may play an important role in increased BBB permeability in this context. © TÜBİTAK.