Research Information System
Turkish Journal of Biochemistry, vol.45, no.6, pp.859-868, 2020 (SCI-Expanded)
Background: The conjugations of antigenic synthetic peptide sequences with carrier polymers have opened new possibilities for the treatment of diseases. In this study, 135-161 peptide sequence of VP1 capsid protein of Footand- Mouth Disease was cross-linked with P(VP-co-AA) copolymer by covalent conjugation using water-soluble carbodiimide at different ratio of components (γ=5, 7, 9, 11, 15) for the first time in the literature. Materials and methods: Bioconjugates were characterized by gel permeation chromatography and fluorescence spectroscopy to identify occurrences of the conjugates. After characterization, γ=15 bioconjugate was determined as optimum conjugate for immunization studies and IC50 value is calculated as 1.227 mg/mL. By determining the nontoxic range, indirect ELISA were performed to evaluate the immune response elicited in balb/c mice by either peptide or P(VP-co-AA)-peptide bioconjugates (γ=15). Two injections were applied to each group and high immune responses were obtained against γ=15 conjugate compared to free peptide and control. Results and conclusion: At the end of 9-week, the general pattern of immunoreactivity was acquired as γ=15>>peptide> control. Peptide formulated in the conjugated form had higher antibody response than free peptide and control (p<0.01, for all in both cases), this conjugate formulation put forward the adjuvant activity of P(VP-co-AA) polymer.