Nickel resistance in fission yeast associated with the magnesium transport system
Molecular Biotechnology, cilt.32, sa.2, ss.139-145, 2006 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 32 Sayı: 2
- Basım Tarihi: 2006
- Doi Numarası: 10.1385/mb:32:2:139
- Dergi Adı: Molecular Biotechnology
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.139-145
- Anahtar Kelimeler: Metal transport, Mg2+ transport system, Nickel resistance, Schizosaccharomyces pombe
- İstanbul Yeni Yüzyıl Üniversitesi Adresli: Hayır
Özet
We isolated and characterized a nickel (Ni2+)-resistant mutant (GA1) of Schizosaccharomyces pombe. This mutant strain displayed resistance to both Ni2+ and Zn2+, but not to Cd2+, Co 2+, and Cu2+. The growth rate of GA1 increased proportionally with increasing Mg2+ concentrations until 50 mM Mg2+. The GA1 mutation phenotype suggests a defect in Mg2+ uptake. Sequence analysis of the GA1 open reading frame (ORF) O13779, which is homologous to the prokaryotic and eukaryotic CorA Mg2+ transport systems, revealed a point mutation at codon 153 (ccc to acc) resulting in a Pro153Thr substitution in the N-terminus of the CorA domain. Our results provide novel genetic information about Ni2+ resistance in fission yeast. Specifically, that reducing Mg2+ influx through the CorA Mg 2+ transport membrane protein confers Ni2+ resistance in S. pombe. We also report that Ni2+ ion detoxification of the fission yeast is related to histidine metabolism and pH. © 2006 Humana Press Inc. All rights of any nature whatsoever reserved.