Akademik Veri Yönetim Sistemi
26. Ulusal ve 1. Uluslararası Farmakoloji Kongresi , İstanbul, Türkiye, 4 - 06 Kasım 2021, ss.268, (Özet Bildiri)
Therapeutic role of estrogen (17β-estrodiol) with regenerative effects in a model of corneal debridation
Objectives: The cornea is very prone to injury, as it is a part of the eye that is exposed to the external environment and is likely to be damaged for various reasons. Corneal abrasion (scratched cornea or scratched eye) is one of the most common eye injuries. Estradiol (Estro) has been shown in various studies to support wound healing and have anti-inflammatory effects. Corneal wound healing is an important medical problem that needs to be treated. For this purpose, we aimed to evaluate the effect of Estro in the corneal abrasion model. Materials-Methods: Sixteen rats; were divided into four groups as control, ABR (Abrasion), ABR+Estro and Estro. A corneal abrasion model was created by scraping the corneal epithelium of the right eye of rats under anesthesia. Left eyes were considered as controls and no action was taken. Topical 17β estro was instilled into the eyes of the groups receiving estro 4 times a day. Animals were sacrificed 72 hours after administration with 80-100 mg/kg Ketamine/8-10 mg/kg Xylazine anesthesia. Both eyes were taken for histopathological, immunohistochemical and genetic analysis. Results: Histopathologically significant hyperemia, mild hemorrhages and inflammatory cell infiltrations were detected in the ABR group. Interleukin-1β (IL-1β) and tumor necrosis factor-alpha (TNF-α) levels were highest in the ABR group, while VEGF levels were the lowest. Estro treatment reversed these parameters. In addition, keratinocyte growth factor (KGF) and e-cadherin levels in the ABR group decreased compared to the control group. KGF and e-cadherin levels were significantly increased in the ABR+Estro group compared to the ABR group (p = 0.027 and p = 0.002, respectively). Conclusion: Estro regressed inflammation by decreasing IL-1β and TNF-α levels, and contributed to supporting the proliferation phase of wound healing by increasing KGF and e-cadherin levels. In this way, it has made positive contributions to the epithelialization of the eye. Therefore, it has the potential to be an ideal drug candidate in case of corneal abrasion in the clinic. In the future, the efficacy of Estro can be evaluated more comprehensively in other eye pathologies or in various studies based on wound healing. Keywords: Abrasion, Estradiol, Inflammation, Cornea, e-cadherin