Akademik Veri Yönetim Sistemi
Experimed, cilt.11, sa.1, ss.38-44, 2021 (Scopus)
Objective: In vitro evaluation of implant materials' effects on cell adhesion and viability can provide useful information for predicting implant biocompatibility. Therefore by using a simple and inexpensive method, it was aimed to investigate whether different implant surface-features might have distinct effects on the viability and adherence of the cells. Material and Methods: Different dental implant surfaces (anodized (AN), blasted wrinkled (BW), grit/acid etched (GA), and hydroxylapatite sprayed (HB)) were tested for their possible effects on adhesion and viability of the adherent human osteoblast cells by using an agar-based in vitro technique. Viability of the cells was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and trypan blue staining. Results: The rate of cell adhesion did not seem to be significantly affected by the differences in surface features of dental implants (AN:78.21±0.52; BW:78.22±0.48; GA:78.44±0.85; HB:77.26±0.96). The surface features of the dental implants had an impact on the viability of the attached cells on the implants. Viability of the attached cells was significantly higher on AN, BW, GA surfaces when compared to the HB surface (AN: 72.28±6.04, BW: 67.02±3.47, GA: 85.82±5.05, and HB: 27.98±10.47). Conclusions: In vitro findings suggests that AN, BW, GA surfaces may provide a better platform than HB surfaces to maintain the viability of bound cells.