Functional groups of elongation factor 2 involved in interactions with guanosine nucleotides and ribosomes

Nurten R., Aktar N. B., Bermek E.

FEBS Letters, cilt.154, sa.2, ss.391-394, 1983 (Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 154 Sayı: 2
  • Basım Tarihi: 1983
  • Doi Numarası: 10.1016/0014-5793(83)80189-6
  • Dergi Adı: FEBS Letters
  • Derginin Tarandığı İndeksler: Scopus
  • Sayfa Sayıları: ss.391-394
  • Anahtar Kelimeler: Amino group specific reagent, Elongation factor 2, Guanosine nucleotide, N-ethylmaleimide, Ribosomal interaction, Sulfhydryl group
  • İstanbul Yeni Yüzyıl Üniversitesi Adresli: Hayır

Özet

Treatment of rat liver EF-2 with N-ethylmaleimide (MalNEt) did not affect the direct interactions of the factor with guanine nulceotides or with ribosomes, but inhibited the binding of guanosine 5′-(β,γ-methylene)triphosphate (GuoPP(CH2)P) to the EF-2-ribosome complex. The amino group reactive reagent 2,4,6-trinitrobenzenesulfonate (TNBS), however, inhibited specifically the direct interactions of EF-2 with guanine nucleotides, but not the binding of GuoPP(CH2)P to the EF-2-ribosome complex. The different sensitivities of EF-2 to MalNEt and to TNBS suggested that the binding sites involved in the binary vs. ternary complex might correspond to different conformational states or might even be distinct physical entities. © 1983.