ADP-ribosylation of serum proteins: Evaluation as a potential tumor marker

Albeniz I. Ü., Nurten R., Bermek E.

Cancer Letters, cilt.108, sa.2, ss.239-245, 1996 (Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 108 Sayı: 2
  • Basım Tarihi: 1996
  • Doi Numarası: 10.1016/s0304-3835(96)04421-7
  • Dergi Adı: Cancer Letters
  • Derginin Tarandığı İndeksler: Scopus
  • Sayfa Sayıları: ss.239-245
  • Anahtar Kelimeler: ADP-ribose transport, ADP-ribosylation, CEA, NAD glycohydrolase, Tumor marker
  • İstanbul Yeni Yüzyıl Üniversitesi Adresli: Hayır

Özet

Serum samples from cancer patients revealed elevated levels of in vitro ADP-ribosylation through non-enzymic binding of ADP-ribose to free acceptor sites on serum proteins. Low concentrations of serum ADP-ribose caused by high NAD glycohydrolase activity together with elevated rates of ADP-ribose transport into erythrocytes appeared to account for under-saturation of the acceptor sites on serum proteins. ADP-ribosylation of serum proteins was assessed as an indicator of cancer disease, and an attempt was made to determine the correlation of ADP-ribosylation levels with carcinoembryonic antigen (CEA) values. Based on positive test results for all tumor patients and negative test results for all healthy controls, sensitivity and specificity of ADP-ribosylation as a tumor indicator were estimated as 67% and 95%, respectively. A close correlation appeared to exist with CEA (r = 0.67; P < 0.001). Similarly, the changes in the levels of ADP-ribosylation correlated with the changes in the levels of CEA during the clinical course (r = 0.58; P < 0.05).