Downregulation of MMP-2 and MMP-9 genes in obesity patients and their relation with obesity-related phenotypes

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AKSOYER SEZGİN S. B., BAYOĞLU B., Ersoz F., Sarici M., Niyazoglu M., DİRİCAN A., ...More

Turkish Journal of Biochemistry, vol.47, pp.425-433, 2022 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 47
  • Publication Date: 2022
  • Doi Number: 10.1515/tjb-2021-0124
  • Journal Name: Turkish Journal of Biochemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, EMBASE, Food Science & Technology Abstracts, Directory of Open Access Journals, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.425-433
  • Keywords: adipose tissue, interleukin-10, MMP-2, MMP-9, mRNA levels, obesity
  • İstanbul Yeni Yüzyıl University Affiliated: No

Abstract

Adipose tissue mediates various bioactive molecules and cytokine discharge. The anti-inflammatory cytokine, interleukin-10 (IL-10), has roles in systemic inflammation. Matrix metalloproteinases (MMPs) are endopeptidases implicating in tissue remodeling, and extracellular matrix degradation. Interleukins and MMPs may have specific roles in obesity development. In this investigation, we marked the roles of IL-10, MMP-2, and MMP-9 in obesity and its related clinical phenotypes. Using real-time quantitative polymerase chain reaction (RT-qPCR), also ELISA, IL-10, MMP-2, and MMP-9 mRNA and protein levels were detected respectively in the subcutaneous adipose tissues of 34 patients with obesity and 36 healthy individuals. MMP-2 and MMP-9 gene expression were significantly downregulated in obesity patients compared to controls (p=0.004, p=0.045). Nevertheless, IL-10 was elevated in the obesity group as to controls (p=0.010). MMP-2 mRNA expression was correlated with fasting blood glucose levels (r=0.426, p=0.013) in the patient group. As for protein levels, MMP-2 concentration decreased in patients compared to controls (p=0.001). Moreover, MMP-2 was correlated with BMI (r=-0.411; p=0.022) and weight (r=-0.381; p=0.034) in obesity group. MMP-2, MMP-9, and IL-10 may be related to increased susceptibility to obesity development and its related phenotypes in a sample of Turkish patients with obesity.