Characterization of 5-HT receptors in rat proximal colon

GELAL A., GÜVEN H.

General Pharmacology, cilt.30, sa.3, ss.343-346, 1998 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 30 Sayı: 3
  • Basım Tarihi: 1998
  • Doi Numarası: 10.1016/s0306-3623(97)00096-7
  • Dergi Adı: General Pharmacology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.343-346
  • Anahtar Kelimeler: 5-HT receptors, 5-Hydroxytryptamine (5-HT, serotonin), Proximal colon (rat)
  • İstanbul Yeni Yüzyıl Üniversitesi Adresli: Hayır

Özet

1. In view of its multiple sites of action, we investigated the activity of 5 hydroxytryptamine (5-HT, serotonin) on various potential receptors in the isolated proximal colon of rats. 2. 5-HT induced concentration-dependent contractions of colonic strips (pEC50 = 7.54 ± 0.12). 3. The 5-HT1 receptor agonist, 5-carboxamidotryptamine, induced concentration-dependent contractions (pEC50 = 5.93 ± 0.27); however, neither the 5 HT3 receptor-agonist, phenylbiguanide, nor the 5-HT4 receptor-agonist, renzapride, caused contractions at concentrations as high as 10-4 M. 4. The 5-HT( 1/4 ) receptor antagonist, methiothepin, caused concentraction dependent nonsurmountable antagonism. The 5-HT3 receptor antagonist, tropisetron, inhibited the contractions to a concentration of 5-HT ≤10-6 M. Ketanserin had no effect on responses to 5-HT. 5. Tetrodotoxin and atropine had no effect on responses to 5-HT. 6. We conclude that contractions to 5-HT are mediated by 5-HT(1-like) and probably 5-HT3 receptors that activate tetrodotoxin insensitive mechanisms.