In vitro activity of linezolid, daptomycin and N-acetylcysteine agents on staphylococcus aureus biofilms in the ventriculoperitoneal shunt model

Creative Commons License

KURUOĞLU T., Önger M. E., Turan D., Kuruoglu E.

Turkish Neurosurgery, cilt.29, sa.1, ss.66-71, 2019 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 29 Sayı: 1
  • Basım Tarihi: 2019
  • Doi Numarası: 10.5137/1019-5149.jtn.22470-18.1
  • Dergi Adı: Turkish Neurosurgery
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.66-71
  • Anahtar Kelimeler: Biofilm, Daptomycin, Linezolid, N-acetylcysteine, Scanning electron microscope
  • İstanbul Yeni Yüzyıl Üniversitesi Adresli: Evet

Özet

AIM: To examine the effects of N-acetylcysteine (NAC) alone and in combination with linezolid (LIN) and daptomycin (DAPT) on methicillin-sensitive Staphylococcus aureus (MSSA) biofilm formation. MATERIAL and METHODS: Twelve groups (each containing six molds) of standard ventriculoperitoneal shunts were infected with MSSA. By using microbiological and electron microscopic evaluation methods, NAC was evaluated, alone and in combination with DAPT and LIN, in terms of preventing and eliminating biofilm capacity. The effect of NAC alone and in combination with DAPT and LIN were shown by microbial counts and electron microscopic observation. RESULTS: There was no significant difference in biofilm formation in shunts after different antibiotic treatments. However, the combination of NAC and DAPT had the highest bactericidal effects of all the groups. CONCLUSION: The resistance of bacteria and the dose-dependent effects of antibiotics can be considered.