Akademik Veri Yönetim Sistemi
Journal of Applied Spectroscopy, cilt.92, sa.1, ss.197-201, 2025 (SCI-Expanded)
Cefpodoxime proxetil (CFP) is a third-generation cephalosporins group antibiotic which is an antibacterial beta-lactam agent that is used in the treatment of acute otitis, pharyngitis, tonsillitis and lower respiratory tract infections (e.g. pinomonia and bronchitis). A simple, fast and sensitive spectrophotometric method was developed for the determination of cefpodoxime proxetile in bulk and pharmaceutical preparations. The proposed method was based on the charge transfer interactions of cefpodoxime proxetil as n-electron donor with 7,7,8,8-tetracyan oquinodimethane (TCNQ) as the π-acceptor. In addition, the described method was validated in accordance with the requirements of ICH guidelines. The absorbance was measured at 596 nm. The parameter of linearity, LOD, LOQ, accuracy, precision and recovery were also evaluated. The assay was linear over the concentration range of 3.0–15.0 μg/mL. The correlation coefficient was obtained 0.9997 for CFP. LOD and LOQ values were found to be 0.036 and 0.108 μg/mL, respectively. Recovery values were obtained ranging from 99.97 to 101.3% according to the standard addition method. The performance of the proposed method was evaluated in terms of the student’s t-test and variance ratio F-test to find out the significance of the proposed methods over the reference spectrophotometric method. The developed method was successfully applied for estimation of CFP in a pure form and as a pharmaceutical preparate.