Akademik Veri Yönetim Sistemi
Acta Microbiologica Hellenica, cilt.67, sa.1, ss.15-26, 2022 (Scopus)
Campylobacter jejuni is the most common cause of bacterial food borne disease, but studies on its pathogenesis are still limited. The effects of various mammalian hormones such as, norepinephrine, epinephrine, serotonin, corticosterone, cortisol and cortisone on growth and different virulence mechanisms of Campylobacter were reported previously. In this study, the possible effects of norepinephrine, melatonin, estradiol, progesterone and insulin on growth, adhesion and invasion of C. jejuni were investigated in human adenocarcinoma colon cell (HT-29) line. Moreover, the alterations on biofilm formation on abiotic surfaces and response to stress conditions were investigated. We also aimed to analyze the effects of hormones and C. jejuni, together or separately, on viability of human adenocarcinoma colon (HT-29) cells. Growth alterations were determined spectrophotometrically. Adhesive/invasive bacterial counts were evaluated by colony counting method. Biofilm formation was analyzed using a microtiter plate assay. The alterations of HT-29 cell viability were determined via methyl thiazolyl diphenyltetrazolium bromide assay. According to ourresults, growth of C. jejuniwas decreased in the presence of hormones in a dose and time-dependent manner (p<0.05). Each hormone at all concentrations reduced the adhesion of bacteria (p<0.0001). The invasion of C. jejuni was increased in the presence of progesterone and insulin at all concentrations; which were statistically significant (p<0.0001). The effects of hormones on biofilm formation were variable. Hormones did not affect C. jejuni growth in oxidative and nitrosative conditions (p>0.05). Each hormone and C. jejuni infection have separately decreased the viability of cells after 24 hours incubation (p<0.005). In 48 hours, viability of cells was significantly increased in the presence of low-level estradiol (p<0.0001) and high-level progesterone (p<0.0005). The hormones were found to be significantly more effective on viability of infected HT-29 cells than non-infected cells. In conclusions, these findings once more showed that hormones affect C. jejuni during infectious processes.