Akademik Veri Yönetim Sistemi
Journal of Medical Biochemistry, cilt.45, sa.1, ss.168-176, 2026 (SCI-Expanded, Scopus)
Background: Paraoxonase 1 (PON1) is a calcium-dependent esterase and exerts antioxidant and antiatherogenic properties. Advanced oxidation protein products (AOPP) are a group of carbonylated protein products showing oxidant-mediated protein damage. This study aimed to deter-mine serum PON1 activities and AOPP concentrations in diabetic patients and to evaluate these parameters in terms of their relationships with diabetes mellitus (DM) and related factors. Methods: A total of 93 patients diagnosed with type 2 DM and 30 healthy controls were enrolled in the study. Serum AOPP levels and PON1 activities were measured spectrophotometrically. Other biochemical parameters, includ-ing glucose, total cholesterol, HDL-C, LDL-C, triglycerides, HbA1c and clinical/demographic data, were measured in the routine blood chemistry laboratory and retrieved from patient files. Results: Serum PON1 activity was significantly lower in patients with DM (31.6 [2 1.4 9-48.45] U/mL) compared to controls (41.08 [2 9.0 7-54.35] U/mL) (p= 0.028). Serum AOPP concentration was significantly higher in diabetic patients (584.6 [4 53.8-778.6] pmol/L) than in controls (173.9 [9 8.7 7-224.1] pmol/L) (p < 0.001). PON1 activity negatively correlated with AOPP concentration and positively with serum HDL-1 levels. AOPP concentration positively correlated with age, weight, HbA1c, glucose, total cholesterol, and LDL-C. A PON1 activity cut-off of ^25 U/m L predicted DM with a sensitivity of 36.56% and specificity of 90% (AUC: 0.634, p= 0.028). An AOPP con-centration cut-off of > 340 mmol/L predicted DM with a sensitivity of 89.25% and specificity of 93.33% (AUC: 0.965). Both PON1 (OR: 10.82 1, 95% CI: 1.959 59.778, p= 0.006) and AOPP (OR: 190.068, 95% CI: 20.1 0 2-1 7 9 7.1 4 8, p< 0.001) were independently associated with DM after adjusting for age, sex, and weight. Conclusions: AOPP and PON1 may play a significant role in the development and progression of DM. In particular, serum AOPP concentrations appear to be distinctive among patients with new-onset DM.