A validated spectrophotometric method for determination of formoterol fumarate dihydrate in bulk and dosage form using methyl orange as ion pair reagent ıyon çifti reaktifi olarak kullanılan metil oranj ile formoterol fumarat dihidratın saf ve dozaj formunun valide edilmiş spektrofotometrik yöntemle tayini

TAŞKIN D., Erensoy G., Sungur S.

Marmara Pharmaceutical Journal, cilt.20, sa.3, ss.275-279, 2016 (Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 20 Sayı: 3
  • Basım Tarihi: 2016
  • Doi Numarası: 10.12991/mpj.20162030844
  • Dergi Adı: Marmara Pharmaceutical Journal
  • Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.275-279
  • Anahtar Kelimeler: Formoterol Fumarate dihydrate, Ion pair complex, Methyl orange, Spectrophotometry
  • İstanbul Yeni Yüzyıl Üniversitesi Adresli: Evet

Özet

In this study rapid, simple, accurate and sensitive spectrophotometric method has been developed for the determination of formoterol fumarate dihydrate in bulk and dosage forms. The method is based on the formation of yellow coloured ion pair complex due to the reaction of formoterol fumarate dihydrate (FF) and methyl orange (MO) at pH 4. Ion pair complex has a maximum absorption at 428 nm in chloroform and a linear calibration over the range of 4-20 μg/mL. The slope of the calibration curve was 0.0433, limit of detection was 0.22 μg/mL and limit of quantification was 0.66 μg/mL. The proposed method has been applied to the assay of formoterol fumarate dihydrate commercially available capsules. There was no significiant difference between the results obtained by proposed and reference methods in view of accuracy and reproducibility. No interference was observed from common excipients.