Akademik Veri Yönetim Sistemi
6th International Eurasian Conference on Biological and Chemical Sciences , Ankara, Türkiye, 11 - 13 Ekim 2023, ss.175, (Özet Bildiri)
The distribution of Cucurbitaceae family members is known for tropical and warmer regions. Interspecific variation of the most common members is still represented by limited information. In this study, chloroplast (cp) genome based phylogenetic analysis were carried out in 10 predominating species (Cucumis melo subsp. melo, Lagenaria siceraria, Luffa acutangula, Luffa aegyptiaca, Momordica charantia, Cucumis sativus, Cucurbita argyrosperma, Cucurbita moschata, Cucurbita pepo, and Cucurbita maxima) of the family Cucurbitaceae. Nucleotide sequences and characteristic data of cp genomes were obtained from GenBank. Correlation matrix and principal component analysis (PCA) for numeric and characteristic data were obtained via GraphPad Prism software. Sequence similarity and UPGMA trees were constructed by using MAFFT software. Bayesian topology analysis were carried out using MrBayes software via TRUBA terminal. R Studio was used to obtain PCA graphics for cp genomes. Correlation matrix showed that there was positive correlation between mRNA number and GC% for each species. In PCA analysis, C. melo showed distinction from remaining species by geographic origin, soil characteristics, and the taste. UPGMA tree showed that intraspecific distribution was clear with 3 distinct sub-divisions. The minimum and maximum genetic variation values were recorded as 3.33% and 0.88%, respectively. Bayesian topology analysis and PCA analysis showed that at least three dimensions were present for cp genomes, and PC1 and PC2 were as 51.2% and 28.9%, respectively. In phylogenetic analyses, it was observed that cp genomes can strongly discriminate between genus as in common polymorphism studies; thus, marker genes can be identified in future studies for species distinction in the Cucurbitaceae family.