Long-term l-NAME treatment potentiates the blood-brain barrier disruption during pentylenetetrazole-induced seizures in rats
Life Sciences, cilt.79, sa.1, ss.16-20, 2006 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 79 Sayı: 1
- Basım Tarihi: 2006
- Doi Numarası: 10.1016/j.lfs.2005.12.034
- Dergi Adı: Life Sciences
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.16-20
- Anahtar Kelimeler: Blood-brain barrier, Epileptic seizures, Evans blue, l-NAME
- İstanbul Yeni Yüzyıl Üniversitesi Adresli: Hayır
Özet
We investigated whether the severity of blood-brain barrier disruption caused by pentylenetetrazole-induced seizures is modified by long-term nitric oxide synthase inhibition in rats. Rats were given N-omega-nitro-l-arginine methyl ester (l-NAME), a nitric oxide synthase inhibitor, in drinking water for 4 weeks, and then treated with pentylenetetrazole to induce seizures. Damage to the blood-brain barrier was investigated using Evans blue dye extravasation. Serum nitric oxide concentration was decreased in l-NAME-treated rats (P < 0.01). l-NAME and/or pentylenetetrazole treatments elevated systolic blood pressure of animals (P < 0.01). l-NAME caused an increase in the mortality rate after pentylenetetrazole injection leading to the death of animals at about 15 min after the onset of the seizure. Pentylenetetrazole-induced seizures in rats treated with l-NAME caused a significant increase in Evans blue dye extravasation into cerebral cortex, diencephalon and cerebellum, as compared with seizures evoked by pentylenetetrazole injection to l-NAME-untreated rats (P < 0.01). Data presented here suggest that the degree of blood-brain barrier disruption induced by seizures is more pronounced in long-term nitric oxide deficiency. © 2005 Elsevier Inc. All rights reserved.