Akademik Veri Yönetim Sistemi
Cell Biology International, cilt.39, sa.2, ss.201-209, 2015 (SCI-Expanded)
Oxidative stress can be defined as the increase of oxidizing agents like reactive oxygen and nitrogen species, or the imbalance between the antioxidative defense mechanism and oxidants. Cell cycle checkpoint response can be defined as the arrest of the cell cycle functioning after damaging chemical exposure. This temporary arrest may be a period of time given to the cells to repair the DNA damage before entering the cycle again and completing mitosis. In order to determine the effects of oxidative stress on several cell cycle phases, human erytroleukemia cell line (K562) was synchronized with mimosine and genistein, and cell cycle analysis carried out. Synchronized cellswere exposed to oxidative stresswith hydrogen peroxide (H2O2) at several concentrations and different times. Changes on mitochondriamembrane potential (ΔΨm) of K562 cells were analyzed in G1, S, and G2/M using Rhodamine 123 (Rho 123). To determine apoptosis and necrosis, stressed cells were stained with Annexin V (AnnV) and propidium iodide (PI) for flowcytometry. Changes were observed in the ΔΨm of synchronized and asynchronized cells thatwere exposed to oxidative stress. Synchronized cells in S phase proved resistant to the effects of oxidative stress and synchronized cells at G2/M phase were sensitive to the effects of H2O2-induced oxidative stress at 500μM and above.