In vitro physiological effects of origanum onites L. (lamiaceae) essential oil treatment on human origin cell lines


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Gündogan G. I., Nath E. Ö.

Turkish Journal of Botany, cilt.45, sa.2, ss.158-171, 2021 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 45 Sayı: 2
  • Basım Tarihi: 2021
  • Doi Numarası: 10.3906/bot-2010-40
  • Dergi Adı: Turkish Journal of Botany
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Geobase, Veterinary Science Database, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.158-171
  • Anahtar Kelimeler: Apoptosis, Cancer, Cellular-migration, Origanum onites, Proliferation, Wound healing
  • Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
  • İstanbul Yeni Yüzyıl Üniversitesi Adresli: Evet

Özet

Origanum onites L. belongs to the Lamiaceae family which is commonly used for symptomatic treatment of the diseases of the gastrointestinal, endocrine, and respiratory systems in Turkish ethnobotanical studies and public. In this study our aim was to investigate the physiological effects of O. onites essential oil (OE) on A549 human lung cancer, MIA PaCa human pancreas cancer and HUVEC human endothelial cell lines. Our findings showed that O. onites OE treatment at all concentrations (4.79-191.6 µg/mL, 24 h) can inhibit cell proliferation and induce apoptosis in A549 and MIA PaCa cell lines (P < 0.0001). Although =19.16 µg/mL OE treatment for 24 h did not inhibit cell proliferation in the HUVEC cell lines, higher concentrations of OE (=38.32 µg/mL) significantly inhibited cell proliferation. Apoptotic cell counts were significantly (P < 0.0001) higher in all cell lines at both incubation times (24 h 56.3 ± 7%, 48 h 53 ± 5% for A549, 24 h 44 ± 7%, 48 h 44.7 ± 7% for MIA PaCa, 24 h 48.7 ± 6%, 48 h 49.3 ± 5% for HUVEC). Migration rates were decreased significantly (P < 0.0001) (24 h 64 ± 6%, 48 h 67.2 ± 4% for A549, 24 h 49 ± 5%, 48 h 69.35 ± 5% for MIA PaCa, 24 h 47 ± 5%, 48 h 69.6 ± 2% for HUVEC). Based on these results, OE has been shown to have promising anticancer effects.